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multiphoton microscopy mpm  (Thorlabs)

 
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    Structured Review

    Thorlabs multiphoton microscopy mpm
    Graphical abstract of experimental design for imaging intravital C’Dot uptake and clearance in bone with 2-photon <t>microscopy.</t> A) Bone and osteocytes were exposed to fluorescent nanoparticles through a local subcutaneous injection above the third metatarsal (MT3) bone in a mouse hind paw. After C’Dot incubation, the MT3 was surgically isolated and secured for <t>multiphoton</t> imaging. B-D) Example images of C’Dots in the lacuno-canalicular network are shown. E-F) The high resolution of our imaging system enables subcellular localization. G) A short C’Dot incubation time and high frame averaging allows visualization of the canalicular network. H) Images can be analyzed for clearance kinetics of nanoparticle signal (from previous paper ) as well as subcellular distribution of signal. Scale bars = 10 μm.
    Multiphoton Microscopy Mpm, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiphoton+microscopy+(mpm)/microscope+photon+two/pmc12381294-262-10-15
    Average 86 stars, based on 1 article reviews
    multiphoton microscopy mpm - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Real-time visualization and modulation of endocytic dynamics in osteocytes in vivo"

    Article Title: Real-time visualization and modulation of endocytic dynamics in osteocytes in vivo

    Journal: Scientific Reports

    doi: 10.1038/s41598-025-05735-1

    Graphical abstract of experimental design for imaging intravital C’Dot uptake and clearance in bone with 2-photon microscopy. A) Bone and osteocytes were exposed to fluorescent nanoparticles through a local subcutaneous injection above the third metatarsal (MT3) bone in a mouse hind paw. After C’Dot incubation, the MT3 was surgically isolated and secured for multiphoton imaging. B-D) Example images of C’Dots in the lacuno-canalicular network are shown. E-F) The high resolution of our imaging system enables subcellular localization. G) A short C’Dot incubation time and high frame averaging allows visualization of the canalicular network. H) Images can be analyzed for clearance kinetics of nanoparticle signal (from previous paper ) as well as subcellular distribution of signal. Scale bars = 10 μm.
    Figure Legend Snippet: Graphical abstract of experimental design for imaging intravital C’Dot uptake and clearance in bone with 2-photon microscopy. A) Bone and osteocytes were exposed to fluorescent nanoparticles through a local subcutaneous injection above the third metatarsal (MT3) bone in a mouse hind paw. After C’Dot incubation, the MT3 was surgically isolated and secured for multiphoton imaging. B-D) Example images of C’Dots in the lacuno-canalicular network are shown. E-F) The high resolution of our imaging system enables subcellular localization. G) A short C’Dot incubation time and high frame averaging allows visualization of the canalicular network. H) Images can be analyzed for clearance kinetics of nanoparticle signal (from previous paper ) as well as subcellular distribution of signal. Scale bars = 10 μm.

    Techniques Used: Imaging, Microscopy, Injection, Incubation, Isolation

    Related Articles

    Isolation:

    Article Title: Real-time visualization and modulation of endocytic dynamics in osteocytes in vivo
    Article Snippet: .. Fluorescent signal inside the functionally isolated MT3 was visualized with multiphoton microscopy (MPM) (Bergamo II, Thorlabs) as previously described . .. Briefly, a 20x immersion objective (XLUMPLFLN, Olympus), 1090 nm wavelength excitation, and a > 647 nm long pass filter acquisition were used for observation of Cy5 encapsulated C’Dots.

    Microscopy:

    Article Title: Real-time visualization and modulation of endocytic dynamics in osteocytes in vivo
    Article Snippet: .. Fluorescent signal inside the functionally isolated MT3 was visualized with multiphoton microscopy (MPM) (Bergamo II, Thorlabs) as previously described . .. Briefly, a 20x immersion objective (XLUMPLFLN, Olympus), 1090 nm wavelength excitation, and a > 647 nm long pass filter acquisition were used for observation of Cy5 encapsulated C’Dots.



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    Image Search Results


    Graphical abstract of experimental design for imaging intravital C’Dot uptake and clearance in bone with 2-photon microscopy. A) Bone and osteocytes were exposed to fluorescent nanoparticles through a local subcutaneous injection above the third metatarsal (MT3) bone in a mouse hind paw. After C’Dot incubation, the MT3 was surgically isolated and secured for multiphoton imaging. B-D) Example images of C’Dots in the lacuno-canalicular network are shown. E-F) The high resolution of our imaging system enables subcellular localization. G) A short C’Dot incubation time and high frame averaging allows visualization of the canalicular network. H) Images can be analyzed for clearance kinetics of nanoparticle signal (from previous paper ) as well as subcellular distribution of signal. Scale bars = 10 μm.

    Journal: Scientific Reports

    Article Title: Real-time visualization and modulation of endocytic dynamics in osteocytes in vivo

    doi: 10.1038/s41598-025-05735-1

    Figure Lengend Snippet: Graphical abstract of experimental design for imaging intravital C’Dot uptake and clearance in bone with 2-photon microscopy. A) Bone and osteocytes were exposed to fluorescent nanoparticles through a local subcutaneous injection above the third metatarsal (MT3) bone in a mouse hind paw. After C’Dot incubation, the MT3 was surgically isolated and secured for multiphoton imaging. B-D) Example images of C’Dots in the lacuno-canalicular network are shown. E-F) The high resolution of our imaging system enables subcellular localization. G) A short C’Dot incubation time and high frame averaging allows visualization of the canalicular network. H) Images can be analyzed for clearance kinetics of nanoparticle signal (from previous paper ) as well as subcellular distribution of signal. Scale bars = 10 μm.

    Article Snippet: Fluorescent signal inside the functionally isolated MT3 was visualized with multiphoton microscopy (MPM) (Bergamo II, Thorlabs) as previously described .

    Techniques: Imaging, Microscopy, Injection, Incubation, Isolation